Table 8.
Details about primers of the candidate reference genes used for qPCR analysis *.
| Gene name | Primer sequences (forward/reverse) | Spanned exons | Amplicon size (bp) |
|---|---|---|---|
| ACTB | CCAACCGTGAGAAGATGACC | 2nd | 97 |
| CCAGAGGCGTACAGGGACAG | 3rd | ||
| GAPDH | CTGGCCAAGGTCATCCAT | 7th | 86 |
| ACAGTCTTCTGGGTGGCAGT | 8th | ||
| SDHA | CATCCACTACATGACGGAGCA | 4th | 90 |
| ATCTTGCCATCTTCAGTTCTGCT | 5th | ||
| GYPC | ATCAACATCGCTGTCATTGC | 3rd | 117 |
| CTCGTTGGTGTCCTATGTGC | 4th | ||
| RPL19 | AGCCTGTGACTGTCCATTCC | 2nd | 126 |
| ACGTTACCTTCTCGGGCATT | 3rd | ||
| YWHAZ | AGACGGAAGGTGCTGAGAAA | 2nd | 123 |
| CGTTGGGGATCAAGAACTTT | 3rd | ||
| PGK1 | ACTCCTTGCAGCCAGTTGCT | 3rd | 101 |
| AGCACAAGCCTTCTCCACTTCT | 4th | ||
| HPRT | CCACCCATCTCCTTCATCAC | 4th | 71 |
| TTCTGGGCAGACCTCAAATC | 5th | ||
| TFRC | TTCTGGGCAGACCTCAAATC | 4th | 106 |
| CAGCTTCACGTGGGACATAA | 5th | ||
| G6PD | TGACCTATGGCAACCGATACAA | 10th | 76 |
| CCGCAAAAGACATCCAGGAT | 11th | ||
| B2M | CTGTCGCTGTCTGGACTGG | 1st | 86 |
| TTTGGCTTTCCATCTTCTGG | 2nd |
*
See additional information about primers, including PCR efficiencies [11].