Table 1.

Parameters affecting crystallization process [10,12,14,15].

Physical factors	Chemical factors	Biochemical factors
Temperature	Precipitant type	Sample purity
Pressure	Precipitant concentration	Sample homogeneity
Gravity	pH	Sequence modifications
Magnetic fields	Buffer type	Posttranslational modifications
Electric fields	Ionic strength	Chemical modifications
Dielectric properties	Sample concentration	Aggregation
Viscosity	Metal ions	Proteolysis
Vibrations and sound	Polyions	Sample pI
Time	Detergents	Ligands, co-factors, inhibitors
Equilibration rate	Heavy metals
Nucleants	Small molecule impurities
Methodology	Crosslinkers
Surface of crystallization device	Reagent source
Sample handling	Reagent formulation