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. 2013 Jun 4;14(6):11842–11860. doi: 10.3390/ijms140611842

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© 2013 by the authors; licensee MDPI, Basel, Switzerland

This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).

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LC-PolScope images showing the birefringent retardance (1st, 3rd, 5th row) and orientation (2nd, 4th, 6th row) of CaCO₃ crystals grown in presence of equimolar amounts of basic peptide AS8 (pI 10.6) and three acidic peptides AA8 (pI 3.51), RR8 (pI 4.33), KK8 (pI 4.33) on wrinkle patterned substrate at three different pH values. Peptide stock solutions at different pH values were: 9.66 mM (each AS8 & AA8/RR8/KK8) at pH 7.75; 9.09 mM (each AS8 & AA8/RR8/KK8) at pH 8.2; 6.13 mM (each AS8 & AA8/RR8/KK8) at pH 9.0. Note that the final peptide concentrations during crystallization were 3.22 mM, 3.03 mM, and 2.04 mM for each peptide. Scale bar: 20 μm.