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. 2013 Jun 4;14(6):11842–11860. doi: 10.3390/ijms140611842

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© 2013 by the authors; licensee MDPI, Basel, Switzerland

This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).

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CaCO₃ crystals grown in presence of basic peptide ES9 (pI 9.41, c = 3.33 mM) and three acidic peptides AA8 (pI 3.51; c = 3.33 mM), RR8 (pI 4.33; c = 3.33 mM), KK8 (pI 4.3; c = 3.33 mM) on wrinkle patterned substrate at three different pH values. Peptide stock solutions were 10 mM each. Images were taken in LC-PolScope mode showing the birefringent retardance (1st, 3rd, 5th row) and orientation (2nd, 4th, 6th row). Note that the switch in morphology occurs between pH 8.2 and pH 9.0 for all peptide combinations. Scale bar: 20 μm.