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. 2013 Jun 6;14(6):12107–12122. doi: 10.3390/ijms140612107

Figure 2.

Figure 2

Figure 2

Activation of protein kinase A (PKA) by Eviprostat. (A) Time-dependent effects of Eviprostat on phosphorylation of the vasodilator-stimulated phosphoprotein (pVASP) at serine 157 and CREB. Mesangial cells were exposed to 50 μg/mL Eviprostat or 0.1% dimethylsulfoxide as vehicle control for indicated duration Cellular protein was extracted and subjected to Western blot analysis using specific antibodies for pVASP and pCREB. Equal loading of protein per lane was verified by reprobing the blot with β-actin for loading control. (B and C) Densitometric analysis of pVASP and pCREB in A were shown, respectively. The results were normalized to corresponding internal controls and data were presented as induction relative to the basal level in control (mean ± SE, n = 4). # p < 0.01 vs. control.