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. 2013 Jun 6;14(6):12107–12122. doi: 10.3390/ijms140612107

Figure 5.

Figure 5

Figure 5

Interference of PI3K and MAPK activation by Eviprostat. (A) Inhibition of PDGF-induced p-ERK and p-AKT by Eviprostat. BSMCs were pretreated to 50 μg/mL Eviprostat and 10 μM FSK for 1 h before exposing to 25 ng/mL PDGF for 24 h. The control was treated with vehicle (0.1% dimethylsulfoxide). Cellular proteins were extracted. The levels of p-ERK and p-AKT were determined by Western blot analysis, and total ERK and AKT were used as internal control, respectively. (B and C) Densitometric analysis of p-ERK and p-AKT expression shown in A. The results were normalized to corresponding internal controls, and data were presented as induction relative to the basal level in control (mean ± SE, n = 4). # p < 0.01, * p < 0.05.