Figure 5.

Induction of apoptosis and reduction in cell counts after combined siRNA plus chemotherapy (CT) treatments in EJ28 bladder cancer cells. Cells were transfected with the respective siRNAs for four hours. “CT only” cells were treated with serum-free OptiMEM medium during transfection. Twenty-four hours after transfection start, cells were treated with 2.1 μg/mL cisplatin for 24 h (a,c) or with 0.9 μg/mL mitomycin C for 2 h (b,d). Rate of apoptosis—presented as sum of early and late apoptotic cells—(a,b), as well as cell counts (c,d) were determined 48 h and 72 h after transfection start.