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. 2013 Jun 13;14(6):12401–12410. doi: 10.3390/ijms140612401

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© 2013 by the authors; licensee MDPI, Basel, Switzerland

This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).

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Expression of TNF-α, IFN-γ, IL-2, IL-4 and IL-5 in spleen cells from mice with different concentration of RTB through cytometric bead array immunoassay (CBA) analysis. Splenocytes were stimulated with RTB (1, 10 and 100 μg/mL) in the presence of 2.5 μg/mL ConA for 48 h. The culture supernatants were collected and assessed for cytokine production (TNF-α, IFN-γ, IL-2, IL-5, IL-4) by CBA analysis. Data are presented as the means ± SD of three replicates. *p < 0.01 compared to the control group.