Fig. 5.

Qβ and Qβ por phenotype of infected cells. (a) Infection of XL1-Blue cells with Qβ. Reduction in cellular mass accumulation is observed starting at 30 min with a maximum at 80 min with phage input m.o.i. of 1. A₂ accumulation (◊) was quantified at intervals during infection. The dashed line represents the number of MurA molecules in XL1-Blue. (b) Qβ has a higher released p.f.u. titre after a single infection cycle than Qβ por. (c) A₂ accumulation during infection of XL1-Blue. Loading was normalized to total volume. Samples were taken at the time points listed (min). (d) Qβ por mutants have an earlier lysis phenotype in XL1-Blue-infected cells. Qβ infection (a) reproduced with Qβ por infections. Qβ-only control (×), Qβ A₂WT (•), Qβ A₂L28P (⧫), Qβ A₂D52N (▪) and Qβ A₂E125G (▾).