Table 2. Critical concentration of A2 to induce host lysis.
Level of induction (μM IPTG)* | Lysis | A2 molecules per cell | Time of analysis (min) |
0 | − | nd | nd |
12.5 | − | nd | nd |
25 | − | 2.2×102 | 60 |
50 | − | 2.3×102 | 60 |
100 | + | 3.6×102 | 15 |
100 | + | 4.9×102 | 30 |
1000 | + | 1.4×103 | 15 |
pZE12-A2.