Table 2. Primers used in this study.

Underlined sequences are restriction sites engineered for cloning.

Primer	Sequence (5′ to 3′)		Application
	Forward	Reverse
787 55 and 53	(55) tcataatgtcgctttgcatgcatttcc	(53) actggtaagcttcctgataatcatcttc	5′ fragment for psr mutation
787 35 and 33	(35) agatccaaccaagctttttacaggttc	(33) tcaacttctacaatatcattc	3′ fragment for psr mutation
787C	ttctttagctttaacgcttcctg	cgcaggtcattctcgcttgg	psr complementation
RgpG 55 and 53	(55) actactcgtatgaggtaaagactatg	(53) tctcgcatttgaattctcaactgcc	5′ fragment for rgpG mutation
RgpG 35 and 33	(35) tctcgcatttgaattctcaactgcc	(33) accatccatcataacatgaac	3′ fragment for rgpG mutation
FtsW	gttatcagcaatcttctt	accattactcatagcata	152 bp, qPCR of ftsW
787	gtgaagccgttgattcagtagagg	tgagacatgattgccgacataacc	199 bp, qPCR of psr
GtfB	agcaatgcagccatctacaaat	acgaactttgccgttattgtca	98 bp, qPCR of gtfB
RodA	tatcgtatgctgcgtgtca	ccaactgctgctccaatg	116 bp, qPCR of rodA
GidA	taatcttcttgctcctac	ttactgcttcttcatctt	155 bp, qPCR of gidA
GtfC	atggcgacaatatgatta	cggatgaaggaataagaa	172 bp, qPCR of gtfC
GtfD	tgacttctgttcgttatg	ggttattgctggtaatga	99 bp, qPCR of gtfD
FtsX	ggttgttgtaggttacttat	cttcaagaatcgtctcatt	181 bp, qPCR of ftsX
FtsQ	ttacagacggcagtattg	ttcagcattagaggagttc	128 bp, qPCR of ftsQ
Ldh	ttggcgacgctcttgatcttag	gtcagcatccgcacagtcttc	92 bp, qPCR of ldh