Table 3. Real-time-PCR analysis of selected genes.
Unique ID | Description/putative function* | Fold-difference relative to UA159† | ||
TW14D | TW251 | TW314 | ||
ldh | Lactate dehydrogenase, Ldh | 1.08 | 1.21 | −1.01 |
SMU.787 | Transcriptional regulator, Psr-like protein | 1.37 | nd | nd |
SMU.246 | Glycosyltransferase N-acetylglucosaminyltransferase, RgpG | −4.0a | −2.74a | 1.1 |
gtfB | Glucosyltransferase B | 2.36a | −1.43 | 16.0a |
gtfC | Glucosyltransferase C | 1.17 | −3.96a | 6.13a |
gtfD | Glucosyltransferase D | −1.35 | −3.0a | −1.52 |
SMU.713 | Cell division protein, FtsW | −4.75 a | −2.77a | −1.77b |
SMU.550 | Cell division protein, FtsQ | −2.08a | −4.80a | 4.56a |
SMU.1324 | Cell division ABC transporter, FtsX | −1.94a | −1.78a | −1.08 |
SMU.1003 | Glucose-inhibited division protein, GidA | −2.29a | −2.9a | 1.8b |
SMU.1279 | Cell division protein; shape-determining protein, RodA | 1.09 | −3.23a | 1.69b |
Description and putative function of the selected genes are based upon those in the published Strep. mutans database.
Data presented here are ratios of the levels of expression in the BrpA- and Psr-deficient mutants, TW14D and TW251, and the Psr-deficient and BrpA-down mutant, TW314, relative to those of the wild-type, UA159, with ‘−’ representing downregulation. Superscript a and b indicate statistical difference between the wild-type and the respective mutant at significance level of P<0.001 and 0.01, respectively. nd, Not detectable.