Effect of increasing concentrations of neutralizing interleukin-10 (IL-10) antibody (0.25, 0.5, 1, 2.5 μg/ml) on the concentration of (A) tumor necrosis factor-α (TNF- α) and (B) interleukin-6 (IL-6) in the media collected from HK-2 proximal tubule epithelial cells after activation with lipopolysaccharide (LPS, 10μg/ml) and/or AT2R agonist (C21, 1μmol/L) for 24 hours. Cytokine concentrations in media were measured by ELISA. Data are represented as mean ± SEM. * indicates p<0.05 vs control, # indicates p<0.05 vs LPS treated and $ indicates p<0.05 vs LPS+C21 treated HK-2 cells (n=7).