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. 2013 Jul 12;8(7):e68468. doi: 10.1371/journal.pone.0068468

Figure 2. Enhanced production of IgM antibodies in the MFG-E8−/− mice.

Figure 2

(A) Immunoblot analysis of the MFG-E8+/+ (WT) and MFG-E8−/− (KO) mice sera using the anti-mouse whole IgG Ab that cross-reacts not only with IgG, but also with IgM. Left panel, SDS-PAGE analysis. Right panel, immunoblot analysis. CBB, Coomassie Brilliant Blue. (B) Identification of the immunoglobulin class. The MFG-E8+/+ and MFG-E8−/− mice sera were analyzed by SDS-PAGE followed by an immunoblot analysis using the antibodies against individual immunoglobulins. (C) ELISA analysis of serum IgG and IgM levels in the MFG-E8−/− mice compared to those in the wild-type mice. In panels A and B, the results are representative of at least three experiments with similar results. In panel C, the results represent the means ± SD of three separate experiments performed in duplicate determinations. Asterisks indicate a significant difference between WT and KO groups: **, P<0.01; ***, P<0.001. Differences were analyzed by the unpaired two-tailed Student’s t test.