Figure 8. Effects of MHC II, ICOSL, IL-2 neutralisation on IFN-γ production in the tripartite cultures.

Individually, anti-MHC II (A), anti-ICOSL (B), anti-IL-2 (C) and Orencia (D) did not affect IFN-γ production. However, when all treatments were combined they did reduce the endothelial cell-mediated enhancement of IFN-γ (E). uRBC  =  unparasitised red blood cells. Control  =  no PBMC, uRBC or iRBC. MHC II, ICOSL and IL-2 neutralising antibodies, and Orencia, were incubated with the tripartite cultures for 24 h. Supernates were then harvested and analysed for IFN-γ. Columns represent means of two separate experiments.