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. 2013 Jul 12;8(7):e68611. doi: 10.1371/journal.pone.0068611

Figure 2. MiR-130a suppressed Runx3 expression.

Figure 2

(A–C) Cells were transfected with a miR-130a inhibitor (anti-miR-130a) or a negative control (blank). (A) Runx3 mRNA level measured by real-time PCR (normalized to β-actin), (B–C) Protein expression of Runx3 by Western blotting and respective densitometric measurement results of Runx3, (D) Luciferase reporter assay. The partially complementary miR-130a-binding site found in the Runx3 3′-UTR (or a mutated binding site) was inserted downstream of a luciferase reporter on the psiCHECK™-2 Vector report plasmid and transfected into EPCs with miR-130a mimic or miR-130a inhibitor. *P<0.05 vs. respective scrambled control (blank) groups. #P<0.05 vs. healthy control group. Similar results were obtained in at least three separate experiments and error bars represent the standard deviation from the mean.