Table 1. Oligonucleotides for cloning miR-130a, shRNA and 3′UTRs of target genes.
Primer | Sequence | |
A | miR-130a | F5′- AAAGGATCCGCATCAAGCCCGAAGTAT -3′ |
R5′- AAAGAATTCGAGGCAGTGTCTATCACAAG -3′ | ||
B | Runx3 | F 5′-GGCGTAAGGGAACTCATAAAG-3′ |
R 5′-GGAGGGAAGAAACTACAAGGAC -3′ | ||
C | Runx3shRNA | F 5′- CCGGGGCTAGCAGCATGCGGTATTTCTCGAGAAATACCGCATGCTGCTAGCCTTTTTG-3′ |
R 5′- AATTCAAAAGGCTAGCAGCATGCGGTATTTCTCGAGAAATACCGCATGCTGCTAGCC-3′ | ||
D | Runx3UTR | F 5′-CCGCCCTGGTGGACTCCT-3′ |
R 5′-CCTTCCACACATCTCAGAGTTATAT-3′ |