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. Author manuscript; available in PMC: 2013 Jul 12.
Published in final edited form as: Autophagy. 2008 Jan 18;4(3):322–329. doi: 10.4161/auto.5603

Figure 6.

Figure 6

Detection of autophagosomes by MDC labeling. Transgenic mice expressing mCherry-LC3 were treated with rapamycin and chloroquine as described in Figure 5. One hr before sacrifice, monodansylcadaverine (MDC, 1.5 mg/kg i.p.) was injected. After sacrifice, the heart was removed and prepared for imaging. Shown is a high magnification image (representative of three hearts), demonstrating colocalization of MDC with mCherry-LC3 (arrows). We did not observe MDC staining that did not overlap with mCherry-LC3, although we did observe some mCherry-LC3 puncta that did not colocalize with MDC.