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SDS-PAGE gel electrophoresis for detection of hyaluronidase activity. Venom from male scorpions (1, 3, 5, 7 and 9) and female scorpions (2, 4, 6, 8 and 19) containing 50 μg of protein each were run in gels containing hyaluronic acid, as described in Materials and Methods. The white band at approximately 46 kDa indicates the presence of hyaluronidase activity. Positive control was a venom sample from the spider Brachypelma vagans.
