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. Author manuscript; available in PMC: 2014 Jul 15.
Published in final edited form as: J Immunol. 2013 Jun 14;191(2):837–847. doi: 10.4049/jimmunol.1201716

FIGURE 4.

FIGURE 4

Lamina propria cells mediate protection against acute colitis in the absence of p53. Conditional knock-out mice for p53 in the intestinal epithelium (Trp53IEC-KO, open circles) and controls (Trp53IEC-WT, closed circles) were generated by crossing floxed p53 mice with villin-Cre transgenic mice. (A) Total p53 expression was examined by immunoblotting in intestinal epithelial cells (IEC) isolated from the colon. (B) Mice were treated with DSS. Body weights were recorded daily and are displayed as mean ± SD of the percentage of the initial weight (n=13 to 15 mice/group; analysis by repeated-measures ANOVA showed no significant difference between the groups). (C) Histological inflammation scores and total mucosal ulcerations were determined on colon sections on day 7. Each data point represents one animal, with means shown as horizontal lines (n= 13 to 15 mice/group); analysis by t-test or rank sum test yield no significant (N.S.) difference. (D) Numbers of apoptotic cells were determined by TUNEL staining and counting per high-power field (HPF) under a fluorescence microscope using a 20x lens. Data are mean + SD (significance evaluated by t-test).