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. Author manuscript; available in PMC: 2013 Jul 12.
Published in final edited form as: J Mol Biol. 2011 Jul 29;410(5):863–874. doi: 10.1016/j.jmb.2011.04.024

Figure 2. Chemical probing experiments on HIV initiation complexes with 69nt model HIV1 genomic RNA.

Figure 2

Accessibility of adenosine N1 and cytosine N3 positions to reaction with dimethyl sulfate (DMS) was detected in the presence or absence of 1:1 stoichiometry of either pure bovine native tRNALys3 (nat) or T7 RNA polymerase transcript (T7). Reactivity with DMS was detected using primer extension with reverse transcription using a DNA primer in the absence (−) and presence (+) of dimethyl sulfate reaction. Reactions were performed at room temperature as described.