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. Author manuscript; available in PMC: 2013 Jul 12.
Published in final edited form as: J Mol Biol. 2011 Jul 29;410(5):863–874. doi: 10.1016/j.jmb.2011.04.024

Figure 5. 1H-15N TROSY experiments for the HIV initiation complex at 1:1 tRNALys3-HIV RNA stoichiometry.

Figure 5

(A.) Spectrum for initiation complex formed with uniformly labeled 13C, 15N-tRNALys3 and unlabeled HIV-1 69nt RNA. (B.) Spectrum for initiation complex formed with uniformly labeled 13C, 15N-HIV-1 69nt RNA and unlabeled tRNALys3. Spectra were acquired at 800 MHz 1H frequency T1-relaxation optimized TROSY at 25°C. Spectral assignments of the imino resonances as discussed in the text are indicated.