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. 2012 Feb 8;32(6):2121–2128. doi: 10.1523/JNEUROSCI.4752-11.2012

Figure 2.

Figure 2.

Dysmorphic bP23H rod photoreceptors with truncated outer segments identified via GFP fluorescence. Representative confocal micrographs of two different models of retinal degeneration before (top) and after (bottom) degeneration. A, C, bP23H/GFP double-transgenic retinas. GFP was coexpressed in the rods of bP23H tadpoles to label rod photoreceptors. A, Dark-reared bP23H/GFP retina. C, Degenerating bP23H retina after 6 weeks in cyclic light. Note the presence of GFP-positive rod cell bodies with severely truncated outer segments. B, D, iCasp9/GFP double-transgenic retinas. B, iCasp9/GFP retina before induction of apoptosis. D, iCasp9/GFP retina 4 d after administrating 10 mm AP20187. Rod photoreceptors are permanently lost and noticeably absent after drug-induced apoptosis. Sections were stained with Alexa Fluor 555-conjugated WGA to visualize membranes (red) merged with Hoechst nuclear stain (blue). Rod photoreceptors were visualized by GFP expression (green). IS, Inner segment; INL, inner nuclear layer. Scale bar, 10 μm.