Figure 3.

Dysmorphic rhoP23H rod cells are able to regenerate in the dark. A, Dysmorphic rod cells can be observed in the degenerating bP23H retina for up to 6 weeks in cyclic light-rearing conditions. Sections were stained with Alexa Fluor 555-conjugated WGA to visualize membranes (red) merged with Hoechst nuclear stain (blue). Rod photoreceptors were visualized by GFP expression (green). B, Relative density of GFP-positive rods in the central retina over time (n = 3, mean ± SEM). C, bP23H rod cells are able to regenerate outer segments lost after 6 weeks in cyclic light. Cessation of degenerating light signal reverses ROS degeneration. Sections were stained with WGA to visualize membranes (green) merged with Hoechst nuclear stain (blue). Scale bars, 20 μm. WT, Wild type.