Figure 5.

Short-term degeneration of bP23H retinas over a 3 week period as monitored by TUNEL. Tadpoles were allowed to develop for 14 d in complete darkness. At 14 dpf, the animals were moved to cyclic light (12 h light/dark cycle) rearing condition. A, TUNEL-stained (red) retinal sections of animals at 2, 5, 13, and 19 d in cyclic light. TUNEL-positive cells in the ONL are indicated by arrowheads. WGA, Green; Hoechst nuclear stain, blue. Scale bar, 100 μm. B, The number of TUNEL-positive cells in the ONL were counted over the 3 week cyclic light period. Graph represents the number of TUNEL-positive ONL cells per cryosection, averaged over at least three samples per time point (n > 3, mean ± SEM). For each sample, TUNEL-positive cells were counted in three sections obtained from the center of the tissue block. WT, Wild type.