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. Author manuscript; available in PMC: 2014 Jul 15.
Published in final edited form as: J Immunol. 2013 Jun 19;191(2):572–582. doi: 10.4049/jimmunol.1300299

Figure 3.

Figure 3

Depletion of marginal zone phagocytes compromises iNKT cell activation. (A) Frequency of IL-4 producing huCD2+dump-(B220-CD11b-) splenocytes following αGalCer or DMSO vehicle immunization of bone marrow chimeras containing either WT or CD1d−/− B cells as described in Material and Methods. (B) Frequency of huCD2+dump- splenocytes following αGalCer or DMSO vehicle immunization of B6.KN2 (WT) or CD11c-DTR.KN2 (DTR) mice 24 hours after DT or PBS treatment. (C) Confocal images of spleen sections depicting the effects of CL treatment on the SIGNR-1+ marginal zone and CD169+ metallophilic macrophage subsets. (D) Frequency of huCD2+dump- splenocytes following αGalCer or DMSO vehicle immunization of 4get/KN2 mice treated 24 hours prior with either clodronate-loaded or PBS liposomes. (E) Frequency of splenic CD1d-tetramer+ cells under the conditions described in D. (F, G) Absolute number of total CD11c+MHCII+ dendritic cells or CD8+ and CD8− DC subsets 24 hours after PBS or CL treatment. (H) Spleen sections showing the localization of total CD11c+ splenocytes following PBS or CL injection 24 hours earlier. Data shown is representative of two (A–C) or three (D–F) individual experiments with three or more mice per group. Scale bar, 100 μm. Error bars represent standard deviation. * p < 0.05; ** p < 0.001; ns, not significant.