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. Author manuscript; available in PMC: 2014 Jul 15.
Published in final edited form as: J Immunol. 2013 Jun 19;191(2):572–582. doi: 10.4049/jimmunol.1300299

Figure 6.

Figure 6

Non-cognate effector functions of iNKT cells in vivo. (A) Histograms showing CCR7 expression of either CD11b+CD8− (left panel) or CD11b-CD8+ (right panel) dendritic cells from WT or CD1d-deficient mice 12 hours after immunization with αGalCer or DMSO vehicle. (B) Confocal microscopy analysis of spleen sections from WT or CD1d-deficient mice 12 hours after immunization with αGalCer or DMSO vehicle. Scale bar, 200 μm. (C) Frequency of CD45.1+ WT or CD45.2+ CD1d−/− DCs from unimmunized mixed chimeric mice as described in Materials and Methods. (D) CD1d expression by WT or CD1d−/− CD8α+ DCs from mixed chimeras. (E) Graph depicts the CCR7 geometric MFI of WT or CD1d−/− CD8α+ DCs in mixed chimera from DMSO vehicle or αGalCer immunized mice at the 12hr time point. ** p<0.001 (F) Phosphorylation of STAT6 in the total B220+ splenocyte population was assessed by flow cytometry in WT, CD1d−/− and STAT6−-− mice 4 hours after αGalCer immunization. (G, H) Geometric MFI of pSTAT6 (G) and pSTAT1 (H) after DMSO vehicle or αGalCer immunization in indicated groups of mice. All data shown is representative of at least 2 individual experiments with 3–4 mice per group.