Figure 4.

LM11A-31 inhibits proNGF binding to p75 after SCI. The protein extracts from the spinal cord collected at the indicated times were subjected to immunoprecipitation with p75 or NGF antibodies and the associated NGF or p75 in respective immunoprecipitation reactions were detected in Western blotting. Note that while the extent of increase in total p75 and proNGF levels was similar between 0 and 100 mg/kg tissues, the interaction between proNGF and p75 was significantly attenuated at 100 mg/kg. The experiments were repeated >3 times, and representative blots are shown.