Table I.
Affinity measurements of C-glycoside analogues with Vβ8.2 TCR and Vβ7 TCR
| ka (M−1s−1) | kd (s−1) | KD (μM) | KD(eq)a (μM) | t1/2 (s) | |
|---|---|---|---|---|---|
| Vβ8.2 TCR | |||||
| α-GalCer | 1.62×105 ± 9.5×103 | 0.02 ± 4.5×10−4 | 0.13 ±0.01 | 0.31 ±0.02 | 34.65 |
| α-C-GalCer | 2.25×105 ± 4.1×104 | 0.29 ± 0.02 | 2.20 ± 0.73 | 1.95 ± 0.11 | 2.39 |
| α-N-Cyc-Cer | 5.67×104 ± 1.67×104 | 0.37 ± 0.01 | 7.10 ± 1.97 | 3.68 ± 0.27 | 1.87 |
| α-C-alkyne-GalCer | ND | ND | ND | 1.64 ± 0.13 | ND |
| α-1C-GalCer | NB | NB | NB | NB | NB |
| α-C-O-GalCer | NB | NB | NB | NB | NB |
| Vβ7 TCR | |||||
| α-GalCer | 2.31×105 ± 3.15×104 | 0.09 ± 0.003 | 0.38 ± 0.01 | 0.47 ± 0.03 | 7.70 |
| α-C-GalCer | ND | ND | ND | 3.11 ± 0.23 | ND |
| α-N-Cyc-Cer | ND | ND | ND | 4.06 ± 0.10 | ND |
| α-C-alkyne-GalCer | ND | ND | ND | 3.23 ± 0.29 | ND |
| α-1C-GalCer | NB | NB | NB | NB | NB |
| α-C-O-GalCer | NB | NB | NB | NB | NB |
a
steady-state affinity determined in separate experiment (see materials and methods)
NB= No binding; ND=not determined