Figure 1.

Schematic diagram of in vitro BBB models and experimental design. BMECs were seeded on the filter of Costar Transwell® inserts (BMEC monolayer). In pericyte co-culture, brain pericytes were cultured with BMECs using Transwell® insert without direct contact. Following treatment with LPS (10, 50, and 100 μg/mL) for 4 hr, both in vitro BBB models were used for HIV-1 transport studies. Conditioned media from the abluminal chamber of BMEC monolayer was collected for cytokine assay.