Figure 4.

Growth of the Δrsh mutant in minimal medium requires addition of methionine only. B. suis 1330 wild-type (black), B. suis strains Δrsh (red), ΔmetH (blue) and ΔmetH complemented with the metH gene on plasmid pBBR1-MCS (green), were grown in TS medium overnight, washed twice, and diluted 1/500 in GMM in the absence (dashed lines) or presence (solid lines) of methionine. Bacterial growth was measured at λ 600 nm. Both Δrsh and ΔmetH mutants were unable to grow in the absence of methionine and both graphs superpose as a “base line”. +met: addition of exogenous methionine; +pBBR1-MCS-metH: complementation plasmid for ΔmetH mutant.