Table 1. Antibodies used for different experiments in this report.
| Antibody | Vendor | Catalog no. | Applications/working dilution |
|---|---|---|---|
| Rabbit anti-Col IV |
Abcam |
ab6586 |
IHC* (1:100) |
| Rabbit anti-MMP-9 |
Abcam |
ab7299 |
IB (1:1000) |
| Rabbit anti-Colα3(IV) NC1 |
Wong & Cheng (current report) |
Not applicable |
IB (4 μg IgG/ml) |
| Rabbit anti-His |
Roche Applied Science |
04905318001 |
IB (1:1000) |
| Rabbit anti-Flag M2 |
Stratagene |
200470-21 |
IB (1:1000) |
| Rabbit anti-Occludin |
Life Technologies |
71-1500 |
IB (1:200) |
| Rabbit anti-CAR |
Santa Cruz Biotechnology |
sc-15405 |
IB (1:200), IF (1:50) |
| Rabbit anti-JAM-A |
Life Technologies |
36-1700 |
IB (1:150) |
| Rabbit anti-ZO-1 |
Life Technologies |
61-7300 |
IB (1:150) |
| Mouse anti-ZO-1 |
Invitrogen |
33-9100 |
IF (1:50) |
| Rabbit anti-N-cadherin |
Santa Cruz Biotechnology |
sc-7939 |
IB (1:200), IF (1:50) |
| Rabbit anti-α-catenin |
Santa Cruz Biotechnology |
sc-7894 |
IB (1:200) |
| Rabbit anti-β-catenin |
Santa Cruz Biotechnology |
sc-7199 |
IB (1:200) |
| Mouse anti-β-catenin |
Chemicon/EMD Millipore |
MAB2081 |
IF (1:50) |
| Goat anti-actin |
Santa Cruz Biotechnology |
sc-1616 |
IB (1:200) |
| Goat anti-rabbit IgG-Alexa Fluor 555 conjugated |
Invitrogen |
A21429 |
IF (1:250) |
| Goat anti-mouse IgG-Alexa Fluor 488 conjugated | Invitrogen | A11029 | IF (1:250) |
*
, IHC, immunohistochemistry; IB, immunoblotting; IF, immunofluorescence microscopy. It is noted that antibodies used for various experiments in this report cross-reacted with the corresponding proteins in the rat as indicated by the manufacturers.