Table 1.
Strains, plasmids and bacteriophage
| Strain, plasmid or phage | Description | Source or reference |
|---|---|---|
| Strain | ||
| TRMG1655 | MG1655 csrA::kan | 43 |
| CF7789 | MG1655 ΔlacI-Z(MluI) | Michael Cashel |
| TRBW3414 | BW3414 csrA::kan, rpoS | 43 |
| PFLA881 | CF7789 PlacUV5-moaA‘-’lacZ | This study |
| TR PFLA881 | PFLA881 csrA::kan | This study |
| PFA279 | CF7789 moaA‘-’lacZ | This study |
| TR PFA279 | PFA279 csrA::kan | This study |
| S17-1 λpir | λ(pir) hsdRpro thi; chromosomally integrated RP4-2 Tc::Mu Km::Tn7 | 56 |
| BW25142 |
laclqrrnB3ΔlacZ4787 hsdR514 DE(araBAD)567 DE(rhaBAD)568ΔphoBR580 rph-1 galU95ΔendA9 uidA(ΔMluI)::pir(wt) recA1 |
57 |
| Plasmids | ||
| pBR322 | Cloning vector, Ampr Tetr | 58 |
| pCRA16 | csrA in blunt-ended VspI site of pBR322 | 12 |
| pLFT | Vector for translational fusions, Ampr | 14 |
| pPFINT | Vector for integration, Tetr | 14 |
| pUV5moaA | pLFT ϕ (PlacUV5-moaA‘-’-lacZ) | This study |
| pmoaA | pLFT ϕ (moaA‘-’lacZ) | This study |
| pCSB12 | csrA in NdeI and BamHI sites of pET21a+ | 5 |
| pCSB12 X#C | csrA X#C C-terminal His6-tag, derived from pCSB12 | This study |
| pCSRH6-19 | pKK223-3 expressing C-terminal His6-tag CsrA, Ampr | 4 |
| pCSRH6-19X#C | csrA X#C C-terminal His6-tag, derived from pCSRH6-19 | This study |
| pAlt-C4 | 48 | |
| pAltermoaA | moaA (LPF85:85) in EcoRI and BamHI sites of pAlter-1 | This study |
| pAltermoaA (109:110) #2 | pAltermoaA with mutations in moaA CsrA binding site BS2 | This study |
| pAltermoaA (111:112) #1 | pAltermoaA with mutations in moaA CsrA binding site BS2 | This study |
| pAltermoaA (113:114) #2 | pAltermoaA with mutations in moaA CsrA binding site BS1 | This study |
| Bacteriophage | ||
| P1 vir | Strictly lytic P1 for generalized transduction59 | Carol Gross |