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. Author manuscript; available in PMC: 2014 Jan 11.
Published in final edited form as: Oncogene. 2012 Sep 3;32(28):3329–3338. doi: 10.1038/onc.2012.344

Figure 3. In vitro demethylation and expression of selected genes in the lung cancer cell line H1299.

Figure 3

A Dots summarize the average methylation and B normalized fold expression for three replicates (treated vs untreated) of 3 genes in the lung cancer cell line and H1299 without treatment, and following treatment with 0.5 μM of 5-aza-2′-deoxycytidine (5-Aza-dC). LINE1 repeat DNA methylation serves as a marker for the efficiency of 5-Aza-dC treatment. Robust bisquare M-regression was used to explore changes in methylation and expression after 72 h 5-Aza-dC-treatment.

Stars indicate significance for changes in methylation;* Pval=0.002, **Pval=0.0006

*** Pval<0.0002

Stars indicate significance for changes in expression; *Pval<0.05