Figure 3. Categorization of Peregrine and ScriptSeq library reads mapping to different RNA species comprising the E. coli K-12 transcriptome. Libraries were prepared from E. coli K-12 total RNA. Each library was sequenced, and reads aligning to the reference E. coli genome were categorized according to the type of RNA species to which they mapped. Additional read statistics for the libraries are included in Table 1; Tables S1 and S2. Left panel: Peregrine libraries were untreated (two replicates), normalized via DSN treatment (four replicates) or prepared from Ribo-Zero treated RNA (four replicates). Right panel: ScriptSeq libraries were untreated (three replicates) or prepared from Ribo-Zero treated RNA (three replicates).