Table 2. Strand specificity of Peregrine libraries prepared from human PBMC RNA.
Treatment | rRNA | CDS |
---|---|---|
Untreated |
99.50 ± 0.28 |
96.37 ± 0.00 |
DSN | 99.71 ± 0.15 | 97.03 ± 0.00 |
Peregrine libraries were prepared from human PBMC total RNA, sequenced, and aligned to the reference human genome. Read statistics for the libraries analyzed are included in Table 2; Table S5. Reads mapping to rRNA genes or CDS were assessed for strand specificity. Values indicate the proportion of reads mapping to the sense strand (mean ± standard deviation).