TABLE 1.

FIV subtype distribution for 68 Serengeti lions based on gene sequence analysis

Liona	Pride affiliation	Year of sample	FIV type(s) detected at indicated gene regionb
			pol-RT (337 bp)			pol-RNase (730 bp), universal primer	gag (444 bp), universal primer
			Primer type A	Primer type B	Primer type C
Ple-531	BARAFU	1989	-	B	-	B	-
Ple-583	BARAFU	1994	-	B	-	B	B
PLE-241	CAMPSITE	1985	-	B	C	B	cB, cC
PLE-254	CAMPSITE	1985	-	B	C	cB	cB, cC
PLE-262	CAMPSITE	1994	A	B	C	cB, cC	cB, cC
PLE-272	CAMPSITE	1986	-	B	C	cB	cB
PLE-276d	CAMPSITE	1991	-	-	C	B	B
PLE-355	CAMPSITE	1987	-	-	C	-	C
PLE-923	CAMPSITE	1998	-	-	C	-	C
PLE-268	CS-NOMAD	1994	-	B	C	cB	cB, cC
PLE-373	GOL UNITED	1994	-	B	-	B	B
PLE-593	GOL UNITED	1994	-	B	-	B	B
PLE-608	GOL UNITED	1996	-	B	-	B	B
PLE-609	GOL UNITED	1994	-	B	-	B	-
PLE-385	KIBUMBU	1987	A	B	-	cB	cA, cB
PLE-388	KIBUMBU	1994	-	-	-	-	-
PLE-389	KIBUMBU	1986	-	B	C	cB, cC	B, C
PLE-916	KIBUMBU	1998	-	B	-	-	-
PLE-342d	LOLINDO	1987	-	-	C	-	B
PLE-458	LOLINDO	1997	-	B	-	-	-
PLE-597	LOLINDO	1994	A	B	-	cB	cB
PLE-610	LOLINDO	1994	-	B	C	-	-
PLE-336	MAASI	1987	-	-	-	B	B
PLE-611	MAASI	1994	-	B	-	B	B
PLE-612	MAASI	1994	-	B	C	cB, cC	cB, cC
PLE-613	MAASI	1994	-	B	-	-	-
PLE-614	MAASI	1994	-	B	-	B	B
PLE-475d	MAASI KOPJE	1986	-	-	C	B	-
PLE-486	MAASI KOPJE	1984	-	B	-	B	-
PLE-603	MAASI KOPJE	1994	-	B	-	B	B
PLE-605	MAASI KOPJE	1994	-	B	-	B	B
PLE-606	MAASI KOPJE	1994	-	B	-	B	B
PLE-591	NAABI	1994	-	B	C	B	B
PLE-598	NAABI	1994	-	-	-	-	B
PLE-599	NAABI	1994	-	B	-	B	B
PLE-601	NAABI	1994	-	B	C	cB	cB, cC
PLE-513	PLAINS	1994	A	B	-	cB	cA, cB
PLE-622	PLAINS	1994	-	-	-	-	-
PLE-623	PLAINS	1994	-	B	-	B	cB
PLE-624	PLAINS	1994	-	-	C	C	cC
PLE-924	PLAINS	1998	A	-	-	-	-
PLE-225	SANGERE	1987	-	B	-	B	-
PLE-506	SANGERE	1993	-	B	C	-	-
PLE-618	SANGERE	1994	-	-	C	cB, cC	cB, cC
PLE-619d	SANGERE	1994	-	-	C	B	B
PLE-621	SANGERE	1994	-	-	C	C	C
PLE-584	SIMBA	1994	-	B	-	B	cB
PLE-630	SIMBA	1994	-	B	-	B	B
PLE-650	SIMBA	1994	-	B	-	-	B
PLE-626	SIMBA EAST	1994	A	-	C	-	-
PLE-627	SIMBA EAST	1994	-	B	-	-	-
PLE-631	SIMBA EAST	1994	-	-	-	-	B
PLE-648	SIMBA EAST	1995	-	B	C	cB, cC	cB, cC
PLE-592	SIMBA WEST	1994	-	-	-	-	-
PLE-346	TRANSECT	1994	-	B	C	cB	cB
PLE-349	TRANSECT	1987	-	B	-	B	B
PLE-350	TRANSECT	1987	A	B	C	cB	cA, cB, cC
PLE-569	TRANSECT	1994	-	B	C	cB	cB, cC
PLE-633	TRANSECT	1994	-	B	C	cB, cC	cC
PLE-634	TRANSECT	1994	-	B	C	cB, cC	cC
PLE-636	TRANSECT	1994	-	B	C	cC	cB cC
PLE-637	TRANSECT	1994	-	B	C	cB	cB
PLE-913	unknown	1997	-	-	-	-	A
PLE-914	unknown	1997	-	-	-	C	C
PLE-915	unknown	1997	-	B	-	B	B
PLE-941	unknown	1997	-	B	C	-	-
PLE-942	unknown	1999	-	B	C	B	B, C
PLE-943	unknown	1999	-	B	C	-	-

^aSample collection and pride designation are by C. Packer and M. E. Roelke (see Fig. 1B).

^bLetters refer to PCR product with sequence that is defined as FIV-Ple A, FIV-Ple B, or FIV-Ple C based on clade position in Fig. 3. Bold type indicates a reference sequence (demonstrates consistent single strain infection). Dash indicates absence of a PCR product with the indicated primer pair.

^cSequences obtained from cloned PCR products. Others are direct sequences.

^dInconsistent results at different loci.