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. 2013 Mar 27;10(4):540–552. doi: 10.4161/rna.24133

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Copyright © 2013 Landes Bioscience

This is an open-access article licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported License. The article may be redistributed, reproduced, and reused for non-commercial purposes, provided the original source is properly cited.

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graphic file with name rna-10-540-g5.jpg — Figure 5. Phenotype tests of the wt and mutant pLAI constructs. (A) CA-p24 production in 293T cells transfected with pLAI variants. (B) LTR-luc constructs for wt and Nef1 were tested for promoter activity in transfected 293T cells. (C) Virus replication in SupT1 T cells monitored by CA-p24 production. Cells were transfected with the indicated constructs at day 0 (x-axis: days post-transfection). (D) Virus replication in PBMCs monitored by CA-p24 production. Cells were infected with the indicated HIV-1 variants at day 0 (x-axis: days post-infection) (left: 0.1 ng CA-p24, right: 1 ng CA-p24).