Table 2. Oligonucleotides used for mutagenesis, PCR amplification and sequencing.

Name	Positiona	Sequenceb
POL1 fw	2651–2696	CAAAATCCAGACATTGTAATATACCAATACATGGATGATTTGTATG
POL1 rev		CATACAAATCATCCATGTATTGGTATATTACAATGTCTGGATTTTG
POL2 fw	2816–2858	GGATGGGTTATGAACTACACCCTGATAAATGGACAGTACAGCC
POL2 rev		GGCTGTACTGTCCATTTATCAGGGTGTAGTTCATAACCCATCC
POL3 fw	3650–3696	CAAGCACAACCAGATAAATCAGAAAGCGAGTTAGTCAATCAAATAAT
POL3 rev		ATTATTTGATTGACTAACTCGCTTTCTGATTTATCTGGTTGTGCTTG
NEF1 fw	8803–8846	CTTTGGATGGTGCTACAAGCTTGTCCCCGTTGAGCCAGATAAGG
NEF1 rev		CCTTATCTGGCTCAACGGGGACAAGCTTGTAGCACCATCCAAAG
FGSA-32-Fb	2289–2308	AAATCC ATACAATACTCCAG
TA051	3471–3488	CAGGGAGACTAAATTAGG
RT-Pfo-as	3973–3997	GTTGCCATATTCCTGGACTACAGTC
5′NEF-1	8277–8300	GCAGTAGCTGAGGGGACAGATAGG
5′NEF-LTR01	8612–8633	CTTTAAGACCAATGACTTACAA
tTA-rev1-AD	8925–8946	GTCAAACCTCCACTCTAACACT

a) HIV LAI

b) Silent mutations marked.