Figure 1. AAGAG/CUCUU repetitive RNAs are components of NuMat. AAGAG and CUCUU transcripts in nuclear and NuMat_RNA were compared for size and abundance. Plasmid DNA with AAGAG insert and 1 kb RNA ladder were loaded as size marker. Northern hybridization with strand-specific probes revealed signal pertaining to either of the strands as specified at bottom of the panels. Lane 1, Nuclear RNA; 2, NuMat_RNA; 3, NuMat_RNA + DNaseI; 4, Plasmid DNA with AAGAG insert; 5, NuMat_RNA; 6, NuMat + RNaseH; 7, NuMat_RNA + RNaseI. Slot blot hybridization shows that no transcripts corresponding to AATAT satellite repeat were present in nuclear or NuMat_RNA. Slot 1, Genomic DNA; 2, Emb nuclear RNA; 3, Emb NuMat_RNA.