Figure 3. Knockdown of AAGAG/CUCUU transcripts is lethal. Transgenic line (pSymp^AAGAG/CyOGFP) was crossed with actinGal4/CyOGFP. GFP positive (CyOGFP/actin-Gal4 or CyOGFP/pSymp^AAGAG) embryo and larvae were wild-type (WT) and GFP negative (pSymp^AAGAG/actin-Gal4) were knockdown (KD). Northern analysis with RNA from WT and KD larvae shows a clear knockdown of AAGAG/CUCUU transcript levels in the RNAi lines when compared with the WT. Loading control is shown by actin hybridization. WT larvae pupate and shrink in length to become mature pupae and hatch to produce flies. KD larvae begin to pupate but stop at early pupal stage, stay at this stage for several days and eventually die.