Figure 3. wgNOESY spectra recorded at 150 ms mixing times and 293 K of wt-ectmRNA 0.2 mM (upper spectrum) and in Δ1-ectmRNA 50 μM (lower spectrum). Upper: a strong cross-peak connects a methyl group and an H6 proton. T₃₄₁H3 connectivity pathway to G₁₃ is similar to that linking wt-TLDaa T₃₈H3 to G₁₂H3. Lower: the methyl resonance and the linked cross-peaks are absent.