Figure 4. Superposition of TLD-Haa jrNOESY spectra recorded at 298 K at 100 and 150 ms mixing times: imino proton region (left) and methyl proton region (right). The pattern of the TLDaa cross-peaks and its assignment is transposable to these spectra, showing that the TLD parts of both RNAs fold similarly. The spectra disclose two methyl proton resonances (boxed cross-peaks), one arising from the expected m⁵U₁₂₁ and the other probably from m⁵U₉₇.