FIG. 1.

Persistence of virus-specific CD8⁺ T cells at high stable memory levels in nonlymphoid tissues during an acute viral infection. Analyses were performed to correlate the kinetics of virus replication (A and D) with the kinetics of virus-specific CD8⁺ T-cell responses (B, C, E, and F). C57BL/6 mice were infected with 10² PFU of LCMV-Docile, and virus titers in different tissues were measured at the indicated times. Data shown are means ± standard errors of the means (SEM) of log₁₀ PFU/g of tissue for 5 to 10 mice. Parallel total numbers of GP133-41 or NP396-404 peptide-specific CD8⁺ T cells were determined by staining with H-2D^b tetramers (•) or measuring intracellular IFN-γ (○) production after the stimulation of cells with the appropriate peptide. Values were derived by multiplying the percentages of total tetramer-positive cells by the total numbers of lymphocytes isolated from a given tissue. Data shown are means ± SEM of log₁₀ virus-specific T cells per spleen for 5 to 10 mice.