FIG. 13.

Rapid functional loss and physical elimination of virus-specific CD8⁺ T cells in lymphoid versus nonlymphoid tissues in the absence of CD4⁺ T-cell help during chronic LCMV infection. C57BL/6-CD4^−/− mice were infected with 2 × 10⁶ PFU of LCMV-Docile, and virus titers in different tissues were measured at the indicated times (A and D). Data shown are means ± SEM of log₁₀ PFU/g of tissue for 3 to 5 mice. Total numbers of GP133-41 or NP396-404 peptide-specific CD8⁺ T cells were determined by staining with H-2D^b tetramers (•) or measuring intracellular IFN-γ (○) production after stimulation of cells with the appropriate peptide (B, C, E, and F). Data shown are means ± SEM of log₁₀ virus-specific T cells per spleen for 5 to 10 mice.