FIG. 3.

Kinetics of total virus-specific CD8⁺ T-cell response in different tissues based on tetramer versus intracellular IFN-γ secretion after stimulation of lymphocytes with DC2.4 virus-infected cells during acute or persistent infections. C57BL/6 mice were infected with 10² (A) or 2 × 10⁶ (B) PFU of LCMV-Docile, and total numbers of virus-specific CD8⁺ T cells (sum of GP133-41 and NP396-404 peptide-specific T cells) were determined by staining with D^b/GP133-41 and D^b/NP396-404 tetramers (•). The total numbers of virus-specific CD8⁺ T cells from tissues were tested for the ability to produce IFN-γ after short-term culturing with virus-infected DC2.4 cells on the indicated days after infection (○). Data shown are means ± SEM of log₁₀ virus-specific T cells per tissue for 3 to 6 mice.