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. 2004 Apr;78(7):3210–3222. doi: 10.1128/JVI.78.7.3210-3222.2004

FIG. 5.

FIG. 5.

In vivo analysis of 3′ processing in the absence or presence of DKAs. (A) Reverse transcription products that were detected by the RNA probe that hybridizes to the plus-strand of HIV-1 DNA (thick line) after HindIII digestion. The fragment of plus-strand strong-stop DNA that was detected by the probe was 122 nt in length (labeled +SSS). Unprocessed DNA generated a 105- or a 106-nt fragment (see text). The DNA ends that underwent the 3′-processing reaction carried out by HIV-1 IN generated a 103-nt product. RNA primers (PPT and tRNA) are shown as a dashed line. Minus-strand DNA is shown as a thin line. (B) Representative electrophoretic analysis of DNA products during HIV-1 infection. The lanes are labeled according to the drug treatment during infection. The sizes of the processed and unprocessed bands were determined by the migration of oligonucleotide markers of 103 and 105 nt length as described in Materials and Methods (results not shown). The band derived from plus-strand strong-stop DNA is labeled as +SSS, the unprocessed band is labeled as un, and the 3′-processed band is labeled as proc.