FIG.7.

Sequencing analysis of 2-LTR-circle junctions. The normal 2-LTR-circle junction is indicated as wild type (WT). GT and AC terminal nucleotides that were removed by IN during the 3′-processing reaction are shown in bold. Dashes represent deletions. All sequences containinga deletion or mutation are aligned to the WT sequence. All sequences containing an insertion are aligned to each other. PPT and PBS sequences are underlined. Insertions containing PBS sequences are adjacent to the 3′ LTR. Insertions containing PPT sequences are adjacent to the 5′ LTR. Large deletions or insertions are indicated by − or + followed by the numbers of total nucleotides deleted or inserted, respectively. The total number of each detected sequence is shown for wild-type virus in the absence of drug treatment (No drug), for D64E mutant of HIV-1 IN in the absence of drug treatment, and for wild-type virus in the presence of monosubstituted and disubstituted DKAs. The total number of sequences that were analyzed is also indicated in the bottom of the table.