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. 2013 Jul 14;19(26):4155–4165. doi: 10.3748/wjg.v19.i26.4155

Figure 3.

Figure 3

RNA interference-mediated inhibition of CDX2 decreased survivin, cyclin D1 and c-Myc mRNA and protein expression. mRNA expression levels of survivin (A), cyclin D1 (C), and c-Myc (E) were determined by semiquantitative reverse-transcriptase polymerase chain reaction. Protein expression levels of survivin (G), cyclin D1 (I), and c-Myc (K) were determined by western blotting. mRNA results were expressed as the ratio of survivin (B), cyclin D1 (D), and c-Myc (F) to glyceraldehyde 3-phosphate dehydrogenase (GAPDH). Western blotting results are expressed as the ratio of optical density of survivin (H), cyclin D1 (J) and c-Myc (L) bands to GAPDH bands. All values are mean ± SE. aP < 0.05 for SGC7901/DDP + small interfering RNA (siRNA)-CDX2 cells vs SGC7901/DDP + siRNA-NC cells and SGC7901/DDP cells. Lane 1: SGC7901/DDP + siRNA-CDX2 cells; Lane 2: SGC7901/DDP + siRNA-NC cells; Lane 3: SGC7901/DDP cells.