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Immunofluorescence of E15.5 lung bud sections at 20X magnification. Endothelial cells were stained with anti-CD31-FITC (green), and lymphatic endothelial cells were localized with anti-LYVE-1-Cy3 (red). Lymphatic endothelial cells can be identified by colocalization of LYVE-1 and CD31 (yellow). Lung lymphatics are identified in the mesenchyme surrounding the airway epithelium. Lymphatics are hyperplastic in N/B+CDH lung buds in comparison to normal and N/B-CDH lung buds.
